The Mafia Did Not Kill John F. Kennedy - 1153 Words

Since the death of John F. Kennedy many theories have evolved regarding the individual or individuals who killed the president. Conspiracy theorist, as well as average citizens, believes Lee Harvey Oswald did not kill the president, or that he was not the only person involved in the assignation. According to a 2005 poll 35.90% of the respondents believe someone other than Lee Harvey Oswald killed President Kennedy (http://forums.armageddononline.org/index.php) View Poll Results: Who killed JFK? Lee Harvey Oswald 13 16.46% Lee Harvey Oswald and someone else 12 15.19% Someone else 29 36.71% Other 9 11.39% The bullet, DUH! 16 20.25% Voters: 79. You have already voted on this poll Possible suspects†¦show more content†¦Many more connections with President Kennedy and the Mafia exist, however the Mafia did not kill President Kennedy. The Mafia had the motive and the resources to kill the president (Anders). They also could have easily concealed their involvement in the assignation. For years prior to the election of President Kennedy the CIA had the phones of various Mafia leaders tapped (Dallek). On all of the recordings held by the CIA there is no evidence of the Mafia’s involvement with the assignation of the president. It is believed the Mafia had more reason to kill the Attorney General Bobby Kennedy, who was assassinated in 1968, than his brother the president. According to Anders â€Å"†¦it’s hard to imagine that a group as corrupt as the organized crime syndicate could have kept this secret for 30 years without a rapid wholesale slaughter of everyone connected to the killing†¦Ã¢â‚¬  Members of the Mafia were investigated and interrogated over the years. Not one individual implicated the Mafia. Although Dallek states in his book the Mafia helped to elect Kennedy president there is evidence this did not happen. In his 2006 article Wood discussed an analysis of the 1960 vote totals from the Mafia controlled election districts in Chicago. It was found individuals in those areas did not show a significant increase in votes for Kennedy. The election totals for West Virginia have also been questioned over the years. The Warren Commission concluded thereShow MoreRelatedAssassination Of Jfk. In The Assassination Attempt On John1345 Words   |  6 PagesAssassination of JFK In the assassination attempt on John F. Kennedy, the 35th President of the United States of America, on 22 November 1963 in Dallas, Kennedy (1917-1963) was fatally shot by Lee Harvey Oswald. As a suspect, Lee Harvey Oswald (1939-1963) was arrested and killed two days later by the nightclub owner Jack Ruby (1911-1967) in police custody. Kenny s successor, Lyndon B. Johnson, the Warren Commission concluded that Oswald was the only culprit. A later convicted investigativeRead MoreThe Kennedy Assassination Conspiracy Theories Essay1055 Words   |  5 Pagesof John F. Kennedy is one of the most controversial and debated topics in American History. JFK was one of the most beloved presidents of our time. One article of his death wrote, The day the country cried. Unlike previous presidential assassinations, the JFK assassination is one that is filled the conspiracy theories. Such theories include a Government cover-up, Mafia influence and Cuban President Fidel Castro. The idea of a lone mind, Lee Harvey Oswald, plotting to kill President Kennedy is tooRead MoreEssay The Assassination of John F. Kennedy718 Words   |  3 PagesAmerican History is the assassination of John F. Kennedy. JFK was one of the most loved presidents of our time. One article of his death wrote, The day the country weeped(John). The JFK assassinati on is filled with many conspiracy theories that cannot be proven to be exact facts. Such theories include a Government cover-up†CIA†, Mafia influence, The Illuminati, and Gay Thrill Kill Theory. The idea that lone mind, Lee Harvey Oswald, plotting to kill President Kennedy is too simple to believe. There’sRead MoreThe Mystery of the JFK Assassination744 Words   |  3 Pagesbaby in August (John F. Kennedy Presidential Library Museum). While the most accepted idea is that he was shot by Lee Harvey Oswald, there are a lot more ideas out there. Some people believe that the Mafia had ties to his assassination, mainly Jack Ruby, or if the CIA or FBI had been involved. Unfortunately, nothing remains except many conspiracy theories. The Kennedy family seemed to have nothing but bad luck afterwards, with most everyone dying. (JFK: 50 Year Anniversary) Kennedy refused to sendRead MoreTo Kill A Kennedy954 Words   |  4 Pagesassassinating President John Fitzgerald Kennedy. Today, the vast majority of Americans believe Oswald’s words, claiming that there was more behind the tragic assassination than the United States government once portrayed. Many have disregarded everything the government had told the world and have come up with their own theories, forming the greatest conspiracy in the history of America, a conspiracy that the world is still butting heads about. With the assassination of President Kennedy, the United StatesRead MoreThe Assassination Of John F. Kennedy1626 Words   |  7 PagesThe Assassination of John F. Kennedy John F. Kennedy, the 35th President of the United States, was assassinated on November 22, 1963 at 12:30 p.m Central Standard Time in Dallas, Texas while riding in a motorcade in Dealey Plaza.[1] Kennedy was fatally shot by Lee Harvey Oswald while he was riding with his wife, Jacqueline, Texas Governor John Connally, and Connally s wife, Nellie, in a presidential motorcade. A ten-month investigation by the Warren Commission from November 1963 to September 1964Read MoreWhy Was The Body Not Seen By The Dallas Coroner?1397 Words   |  6 PagesNevertheless, quite suspicious is how Kennedy s body was placed in a casket and taken from Parkland Hospital and driven to Air Force One, where it was loaded aboard the airplane through the rear door, where it remained at the rear of the passenger compartment, in place of a removed row of seats. The body was removed before the Dallas County coroner Earl Rose, which violated Texas state law (the murder was a state crime and occurred under Texas l egal jurisdiction), could conduct a forensic examinationRead MoreJfk Was A Victim Of A Conspiracy Essay1067 Words   |  5 Pagesmorning of November 22, 1963 many people gathered in downtown Dallas, Texas to see President John F. Kennedy ride through a parade. Many spectators watched as President JFK was shot. Since JFK’s death, 50 years ago, many conspiracy theories have evolved. 73% of all Americans believe that JFK was a victim of a conspiracy (Southwell). The Warren Commission concluded that Lee Harvey Oswald killed John F. Kennedy, and that he acted alone. However, over half of the American population believe otherwiseRead MoreThe Assassination of Kennedy and The Conspiracies Surrounding It1530 Words   |  7 PagesJohn Fitzgerald Kennedy was made president January of 1961. The people of America adored him and his wife, Jacqueline. However, on November 22, 1963, Kennedy was shot and killed while riding in the back of his car through Dallas, Texas. The audience of the parade he was in had to watch in horror as their idol died in front of them. He was 46 years old. We grew being told in school that the man who shot John F. Kennedy was Lee Harvey Oswald. However, as I grew older I started learning moreRead MoreEssay on JFKs Death1722 Words   |  7 Pages by Jack Rubys mafia, with the assistance of the Central Intelligence Agency (CIA) that committed the act of treason, in the murder of John Fitzgerald Kennedy (JFK). There is hard evidence supporting the cover up and scandal involved with his assassination. Due to the confiscation of films and evidence, the citizens of the United States must only know what they are told by the government. The CIA was associated with the mafia and arranged the assassination of John F. Kennedy.

Was the American Civil War Inevitable - 2559 Words

Was the American civil war inevitable? The civil war was inevitable, only however, after one key event; the cotton gin made the civil war inevitable. The invention of the cotton gin in 1793 was the key element which enabled the south to have sufficient vested interest in their traditional lifestyle in order to feel the need to defend it at all costs even from their Northern countrymen. The core argument of this essay centres around the evidence which clearly defines their being in existence two ‘nations with in America constantly in opposition to each other. Therefore the growth of sectionalism and the events which led up to the conflict made war an inevitable outcome of the hostilities which had arisen from the to ideologically†¦show more content†¦Failure to compromise over the issue of protectionism was a primary factor in the growth of sectionalism which necessitated war. The underlying conflicts between North and South were finally fully exposed as a result of failure of compromise in the political arena. The failure of American leadership in 1846-1861was epitomised by key events such as; Douglass Kansas Nebraska act of 1854 and the dread Scott case pronouncement of 1857. Both of these events overturned the previous Missouri compromise and thus once again brought the two opposing nations head to head. The Wilmot proviso bill which proposed to eliminate slavery in the territories was a clear signal to the South that the North was plotting against her way of life. Thus the southern mind set became increasingly locked in a persecution complex which they justified by evidence of a ‘Northern conspiracy to destroy their economic institution, the Wilmot proviso was one such piece of evidence even though it was not passed. The election of Lincoln was the final straw with which the south believed the northern conspirators would gain the upper hand and bri ng about the destruction of the Southern institutions. Most irresponsible, wanton, and disastrous of all was the decision of those southern leaders who in 1858-1860 turned to the provocative demand for Congressional protection of slavery in all the territories of the Republic. Allan Nevins. Nevins in the previous quote demonstrates the reckless extentShow MoreRelatedWas The American Civil War An Inevitable?1390 Words   |  6 PagesSabrina Scovino Was the American Civil War an inevitable consequence of the American Revolution? The American Revolution marked significant changes in the political, social, and economic status of the Americans. For a long, time the lives of the Americans were darkened by the British colonial rule. The British government passed several intolerable Acts. For instance, the Massachusetts Government Act was a restriction to town meetings. As a result, the American opponents began collective actionsRead MoreThe American Civil War Was Inevitable1975 Words   |  8 Pagesitself cannot stand were the words of Abraham Lincoln in a republican convention on June 17,1858 in Illinois. The inevitable debate over slavery, popular sovereignty, the publishing of Uncle Toms Cabin, and Lincolns election would eventually have brothers versus brothers fighting each other in a bloody war. Religion, economics and the lost of power made the civil war an inevitable one. Popular sovereignty is the ideal that people could choose their laws such ideal Lewis Cass first broughtRead MoreThe American Civil War Was Inevitable Essay1641 Words   |  7 PagesThe Civil War was inevitable in many reasons. The economic and industrial evolution was mainly in the North side of the United States while the South was just a cotton kingdom, Slave Empire. Also both were completely opposites of one another when it was about freeing the slaves or hiring more. With many debates there has to be sides that would be separated especially if the president has so much hate from the people. With that being said, since many want opposing ideas, the Civil War becomes muchRead MoreEssay on The American Civil War Was Inevitable1930 Words   |  8 PagesThe Civil War: one of the most pivotal and significant moments in the history of the United States of America. The dividing of a newly birthed nation upon itself - the turmoil created threatened to collapse a unified yearning f or independence. A nation once united by the solace of solidarity, once tread on by the tyranny of a motherland, once triumphant in a fight for freedom, became segregated by principle. Power and greed fueled a dichotomy between color and people which repercussions lingeredRead MoreThe American Civil War Was Inevitable Essay1653 Words   |  7 PagesThe Civil War between the North and South was the result of two cultures that economically, morally, and legally clashed on almost all levels. The steadily growing conflict between the two parts of the union makes it hard to pinpoint the origin or the cause of the resulting war. The conflict arose from a nation thats geographical areas had slowly grown apart in their ideals and also their source of income, which is often the cause of strife between battling regions. This rift driven between the twoRead MoreEssay about The American Civil War Was Inevitable1813 Words   |  8 PagesSouth is truly one of the most defining events in America n history. Through numerous events leading up to the start of the Civil War, I will attempt to show how the United States was destined for conflict and that the Civil War was inevitable. The first way I will show how the war could not be avoided will deal with the issue of slavery. Slavery should be the first mentioned because many conflicts within the United States leading up to the Civil War and the division of the United States dealt withRead MoreThe War Of The American Civil War960 Words   |  4 Pagesslavery even if it meant war caused peace in this nation. Slavery was the vital cause of the American Civil War. The north and the south both had their differences on how to run the country. People in the North believed in unity and that slavery should not exist because â€Å"all men are created equally.† On the other hand, the South believed in continuing slavery. People tried to talk it out and come to a middle ground after both sides compromising, however that didn†™t work and caused war. Ideological differencesRead MoreSlavery as the Cause of the American Civil War Essay1733 Words   |  7 PagesThe American Civil War was the bloodiest military conflict in American history leaving over 500 thousand dead and over 300 thousand wounded (Roark 543-543). One might ask, what caused such internal tension within the most powerful nation in the world? During the nineteenth century, America was an infant nation, but toppling the entire world with its social, political, and economic innovations. In addition, immigrants were migrating from their native land to live the American dream (Roark 405-407)Read MoreThe Civil War Was Inevitable1399 Words   |  6 PagesThe American Civil War took place from April 12, 1861 to May 9, 1865. The simple answer is yes: the Civil War was completely inevitable, but there were many events, documents and people before its beginning that certainly had a large bearing on the war itself. The most divisive political issue in the United States in the mid-1800s was the expansion of slavery, and slavery is certainly the common denominator of the events leading up to the Civil War. People from the North were abolitionists, lookingRead MoreThe American Revolution And The Civil War1567 Words   |  7 Pages In the eyes of most, the American Revolution was a momentous event that shaped not only what would now be the America we know today but the whole world as well. However, this event had ramifications that affected the history of America for many years to come. In fact, many of those same ramifications led to what we know as the American Civil War. While it may be difficult to distinguish whether or not the Civil War was an inevitable consequence of the American Revolution due to lack of correlating

Scholarship Essay Example For Students

Scholarship Essay Scholarship EssayNo greater contribution can be made to a specific field than research that leads to the development of a new and superior technology. Universities and businesses constantly attempt to remain on the cutting edge of new design, manufacturing, and application systems through research and development. If I were given the opportunity to conduct my own research, I would dedicate my efforts to the design of computer processing chips with the goal of increasing their speed and computational ability. It is now possible to construct memory chips almost atom by atom, allowing for nearly infinite circuitry capacity. My research would attempt to design a chip that could process data faster, and with more accuracy, than is presently possible. This chip could then handle more complex software, process data from CDs, and handle Internet applications at a faster rate, allowing for equalization with the increase in speed of CD ROM drives and modems. The procedure for this research would entail first learning as much as possible about the design of present chips, the specific materials that I might want to include as conductors, and which metals are easiest to manipulate. Next I would lay out the chip and attempt to construct it with existing technology. Finally, I would need to test it in an effort to make modifications and improvements in the original design. The computer age presents wonderful opportunities, and any research that promotes the facile use of PC or network systems will help advance this cause. For this reason, I would dedicate my year of research to improvements in the design of central processing units.

Relationship Between Modern Imperialism and Economic Globalization

Introduction Globalization of the economy has played a vital role in shaping the post-war period. Over the last fifty years, international trade in services and goods has increased tremendously. In addition, international financial flows have also increased immensely. The rise of modern imperialism has a close relationship with economic globalization.Advertising We will write a custom essay sample on Relationship Between Modern Imperialism and Economic Globalization specifically for you for only $16.05 $11/page Learn More The two concepts can be linked to each other in various ways. Although globalization may be taken to mean developments that have occurred in the recent past (especially in the period from 1990s), the focus of globalization should be long term since it relates to the formation of the modern world through imperialism (Hepp 40-100). The relationship between economic globalization and modern imperialism Modern imperialism and globalization can be regarded as aspects that came to being with the intention of changing the world as well as interpreting the world. In many international forums, modern imperialism and economic globalization have taken centre stage due to the links, which exist between the two concepts. First, economic globalization relates to modern imperialism in that the origin of the two concepts dates back to 500 years ago. During this time, activities such as European imperialism, which started at a local level, became regional and eventually international, thus contributing to economic globalization. For example, imperialism can be associated with the establishment of a free market, which has become a common phenomenon in the arena of economic globalization. The expansion of the global economy has become a widespread phenomenon as a result of imperialism (Hepp 40-100). Modern imperialism also relates to economic globalization in that imperialism carried out by European nations led to the worldwide spre ad of the spirit of capitalism. This has played a significant role in economic globalization as it led to the establishment of global empires, which started during the 1500s. Modern imperialism also relates to economic globalization in that the European and Western powers emphasized on civilization, as they spread in most parts of the world; this ultimately led to economic globalization. The spread of European imperialism meant that most of the structures in society had to be rearranged. For example, politics and the economy got rearranged through imperialism. As a result, there was the introduction of new technology and new ways of doing things in the corporate world. This has had significant contributions to economic globalization (Fernandez-Armesto 674-685). The interactions between countries, which are enhanced through modern imperialism, contribute to economic globalization. Modern imperialism has also contributed to economic globalization through flows in international trade. As a result of imperialism, financial flows have become common in the economy of the world. (Fernandez-Armesto 674-685). The global economy witnessed significant changes during the 18th century.Advertising Looking for essay on history? Let's see if we can help you! Get your first paper with 15% OFF Learn More During this time, there was widespread trade between the European and Asian countries as rulers from Europe expressed a lot of interest in goods manufactured in Asia. This resulted to trade in goods such as silk, cotton, tea and spices. This trade in goods contributed significantly to the aspect of globalization as it speeded up economic globalization. Modern imperialism has also played a role in ensuring that there is speculation in far as international finance is concerned. As a result, people have purchased assets with the hope that the value of the assets will rise in the future since there is a global economy. The trade avenues provided by modern imperialism ensure that there is a reduction in the cost of financial transactions internationally. Consequently, investors have been able to access international markets, as well as foreign markets, which are outside their home country. This plays a crucial role in enhancing economic globalization, as it has become possible to carry out transactions with other people in various parts of the world (Hepp 40-100). Modern imperialism can be termed as the reason behind the liberalization taking place in financial markets. This liberalization has contributed significantly to financial investment; consequently, this enhances economic globalization. After the end of the Second World War in 1945, there was the establishment of international powers and this altered the international economy. The economic system established after 1945 did away with the systems that existed in the interwar period. Economic globalization spread widely after World War II; this phenomenon had started in the nineteenth centur y and went up to the twentieth century. The economic activities carried out by the developed countries during the post-war era contributed to modern globalization (Attack par. 1-13). Imperialism also relates to the aspect of economic globalization with respect to the formation of political ideologies and blocs after World War II. With each bloc aiming to outperform the other, economic globalization became deeply rooted as a result of the competition between the two blocs. The Soviet Union led bloc mostly comprised of countries in the Middle East and Asia, as well as those in Eastern Europe and it advocated for communism. On the other hand, the bloc led by the United States of America advocated for a capitalist form of economy. Each bloc spread its ideals to various parts of the world, thus contributing to economic globalization. Of most importance was the role played by capitalism in spreading economic globalization. With its advocacy of a free market, capitalism led to economic glo balization in the sense that international trade became a reality (Fernandez-Armesto 674-685).Advertising We will write a custom essay sample on Relationship Between Modern Imperialism and Economic Globalization specifically for you for only $16.05 $11/page Learn More The first phase of globalization is believed to have taken place before the post-war period, with economic globalization being witnessed between 1875 and 1914. The industrialization witnessed during this period made notable contributions to the commercialization of the world economy, thus leading to economic globalization. Imperialism led to the rise of new centres of agricultural and industrial production. As a result, millions of people migrated in search of better jobs and desirable living conditions. This migration of people from European countries to such destinations as United States, Brazil, and Canada transformed the international economic structure (Hepp 40-100). Modern imperialis m led to a sharp rise in economic globalization after the end of the Second World War. Since the war had led to the shattering of the world economy, countries such as the United States and Britain aimed at repairing the international economy. As a result, multinational corporations dominated the production as well as distribution of goods all over the world. Economic globalization took shape with the introduction of modern technology. For example, the advancement of information technologies such as computers led to the ease of access to information through the internet. Other factors that contributed to economic globalization included the invention of the airplane and the telephone (Hepp 40-100). Conclusion While concluding this study, it is worth noting that there exists a strong relationship between economic globalization and modern imperialism. As developed nations aimed at spreading their ideals to the developing world, economic globalization was born. Today, the effects of glob alization can be felt in almost every part of the world. With globalization, international trade has become a reality; farmers in the developing countries have access to international markets where they can market their farm produce. Works Cited â€Å"Attack at Pearl Harbor, 1941,† Eyewitness to History, 1997. Web. http://www.eyewitnesstohistory.com/hiroshima.htm Fernandez-Armesto, Felipe. The World – A Brief History, Combined Volume. New Jersey, NJ: Pearson, 2009. Print.Advertising Looking for essay on history? Let's see if we can help you! Get your first paper with 15% OFF Learn More Hepp, John. Historical Foundations of the Modern World: Imperialism and economic globalization. Pennsylvania: Wilkes University, 2012. Print. This essay on Relationship Between Modern Imperialism and Economic Globalization was written and submitted by user Ulik to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Free Essays on Communication And Race

Communication and Race The most critical aspects of media performance with regards to race and ethnicity are issues that relate to the quality of its representation of the lives of people of color. Accuracy and diversity are aspects of media performance that will allow for a careful evaluation of how it represents people of color. Evaluating media performance is important because of the utilization and reliance on the mass media for much of society's reflexive monitoring and evaluation of reality. The performance of mass media is important in regards to race and ethnicity because the mass media are the primary source of indirect or mediated experiences that reinforce racial attitudes and beliefs that are integrated into cognitive structures (Gandy, 1998). Thus, mass media can be viewed as places where reality is constructed and racism may be reproduced. Accuracy Accuracy is an important aspect of media performance to consider in assessing the quality of its representation of the lives of people of color be cause of its assumed consequences of developing self-image and personal identity and its reflection of social reality that informs social policy (Gandy 1998). Shan and Thorton (1994) state that previous research of minority groups has shown that minorities are sometimes depicted as being "violent, primitive, and politically unsophisticated." Furthermore, they postulate that certain types of behaviors among minorities may be "understood by whites as pathological or deviant" because of how the news media represent them. This representation is inaccurate because it ignores historical context and structural explanations for social trends. In addition, Shan and Thorton state that newsmagazines reproduce racism by perpetuating a racial hierarchy carried out by mainly whites and consumed by mainly whites that positioned blacks at the bottom. Entman (1990) found that local news stimulated the production of modern racism. Violent crimes comm... Free Essays on Communication And Race Free Essays on Communication And Race Communication and Race The most critical aspects of media performance with regards to race and ethnicity are issues that relate to the quality of its representation of the lives of people of color. Accuracy and diversity are aspects of media performance that will allow for a careful evaluation of how it represents people of color. Evaluating media performance is important because of the utilization and reliance on the mass media for much of society's reflexive monitoring and evaluation of reality. The performance of mass media is important in regards to race and ethnicity because the mass media are the primary source of indirect or mediated experiences that reinforce racial attitudes and beliefs that are integrated into cognitive structures (Gandy, 1998). Thus, mass media can be viewed as places where reality is constructed and racism may be reproduced. Accuracy Accuracy is an important aspect of media performance to consider in assessing the quality of its representation of the lives of people of color be cause of its assumed consequences of developing self-image and personal identity and its reflection of social reality that informs social policy (Gandy 1998). Shan and Thorton (1994) state that previous research of minority groups has shown that minorities are sometimes depicted as being "violent, primitive, and politically unsophisticated." Furthermore, they postulate that certain types of behaviors among minorities may be "understood by whites as pathological or deviant" because of how the news media represent them. This representation is inaccurate because it ignores historical context and structural explanations for social trends. In addition, Shan and Thorton state that newsmagazines reproduce racism by perpetuating a racial hierarchy carried out by mainly whites and consumed by mainly whites that positioned blacks at the bottom. Entman (1990) found that local news stimulated the production of modern racism. Violent crimes comm...

Learn About the Sahara Desert

Learn About the Sahara Desert The Sahara Desert is located in the northern portion of Africa and covers over 3,500,000 square miles (9,000,000 sq km) or roughly 10% of the continent. It is bounded in the east by the Red Sea and it stretches west to the Atlantic Ocean. To the north, the Sahara Deserts northern boundary is the Mediterranean Sea, while in the south it ends at the Sahel, an area where the desert landscape transforms into a semi-arid tropical savanna. Since the Sahara Desert makes up nearly 10% of the African continent, the Sahara is often cited as the worlds largest desert. This is not entirely true, however, as it is only the worlds largest hot desert. Based on the definition of a desert as an area receiving less than 10 inches (250 mm) of precipitation per year, the worlds largest desert is actually the continent of Antarctica. Geography of the Sahara Desert Stocktrek Images / Getty Images The Sahara covers parts of several African nations including Algeria, Chad, Egypt, Libya, Mali, Mauritania, Morocco, Niger, Sudan, and Tunisia. Most of the Sahara Desert is undeveloped and features a varied topography. Most of its landscape has been shaped over time by wind and includes sand dunes, sand seas called ergs, barren stone plateaus, gravel plains, dry valleys, and salt flats. Around 25% of the desert is sand dunes, some of which reach over 500 ft (152 m) in height. There are also several mountain ranges within the Sahara and many are volcanic. The highest peak found in these mountains is Emi Koussi, a shield volcano that rises to 11,204 ft (3,415 m). It is a part of the Tibesti Range in northern Chad. The lowest point in the Sahara Desert is in Egypts Qattara Depression at -436 ft (-133 m) below sea level. Most of the water found in the Sahara today is in the form of seasonal or intermittent streams. The only permanent river in the desert is the Nile River that flows from Central Africa to the Mediterranean Sea. Other water in the Sahara is found in underground aquifers  and in areas where this water reaches the surface, there are oases and sometimes small towns or settlements like the Bahariya Oasis in Egypt and Ghardaà ¯a in Algeria. Since the amount of water and topography varies based on location, the Sahara Desert is divided into different geographic zones. The center of the desert is considered hyper-arid and has little to no vegetation, while the northern and southern portions have sparse grasslands, desert shrub and sometimes trees in areas with more moisture. Climate of the Sahara Desert Samere Fahim Photography / Getty Images Although hot and extremely dry today, it is believed that the Sahara Desert has undergone various climatic shifts for the last few hundred thousand years. For example, during the last glaciation, it was bigger than it is today because precipitation in the area was low. But from 8000 BCE to 6000 BCE, precipitation in the desert increased because of the development of low pressure over ice sheets to its north. Once these ice sheets melted, however, the low pressure shifted and the northern Sahara dried out but the south continued to receive moisture due to the presence of a monsoon. Around 3400 BCE, the monsoon moved south to where it is today and the desert again dried out to the state it is in today. In addition, the presence of the Intertropical Convergence Zone, ITCZ, in the southern Sahara Desert prevents moisture from reaching the area, while storms north of the desert stop before reaching it as well. As a result, the annual rainfall in the Sahara is below 2.5 cm (25 mm) per year. In addition to being extremely dry, the Sahara is also one of the hottest regions in the world. The average annual temperature for the desert is 86 °F (30 °C) but during the hottest months temperatures can exceed 122 °F (50 °C), with the highest temperature ever recorded at 136 °F (58 °C) in Aziziyah, Libya. Plants and Animals of the Sahara Desert kristianbell / Getty Images Due to the high temperatures and arid conditions of the Sahara Desert, the plant life in the Sahara Desert is sparse and includes only around 500 species. These consist mainly of drought and heat resistant varieties and those adapted to salty conditions (halophytes) where there is sufficient moisture. The harsh conditions found in the Sahara Desert have also played a role in the presence of animal life in the Sahara Desert. In the central and driest part of the desert, there are around 70 different animal species, 20 of which are large mammals like the spotted hyena. Other mammals include the gerbil, sand fox,  and Cape hare. Reptiles like the sand viper and the monitor lizard are present in the Sahara as well. People of the Sahara Desert Zine Elabidine Laghfiri / EyeEm / Getty Images It is believed that people have inhabited the Sahara Desert since 6000 BCE and earlier. Since then, Egyptians, Phoenicians, Greeks,  and Europeans have been among the peoples in the area. Today the Saharas population is around 4 million with the majority of the people living in Algeria, Egypt, Libya, Mauritania, and Western Sahara. Most of the people living in the Sahara today do not live in cities; instead, they are nomads who move from region to region throughout the desert. Because of this, there are many different nationalities and languages in the region but Arabic is most widely spoken. For those who do live in cities or villages on fertile oases, crops and the mining of minerals like iron ore (in Algeria and Mauritania) and copper (in Mauritania) are important industries that have allowed population centers to grow.

A Doll's House by Henrik Ibsen Research Paper Example | Topics and Well Written Essays - 1250 words

A Doll's House by Henrik Ibsen - Research Paper Example Consequently, it is via these events where people while mingling embrace their effective roles based on genders evident in the play. In this play, the author significantly explores the aspect of feminism in the society with intention of creating senses of social equality in regards to both genders. In this play, act as recipients of both oppression and unfair treatment that makes them appear as men’s play objects. Ibsen illustrates an aspect of mistreatment of women through one of the character Nora, where the male individual oppresses the female especially at the family level (Ibsen 24). Henrik exemplifies oppression on women in the society quite evident at various social, economic and political avenues. However, his focus is at family level where the male gender seems to assumes the top rank whereas are subjects almost close to the children they have given birth. Oppression against the female gender is also evident globally whereby despite the level of development of that given region women end up not receiving economic support. As a result, they have no mediums to air the grievances that can help them to demand liberation or champion the colleagues to embrace liberation. The play thus reveals oppression against women by illustrating the societal perception of women. For instance, the society perceives women as the secondary citizen according to the play, as she depends mostly on a man for her survival and existence. According to the play, a woman should be inferior to a man, and her actions should not override the males’ decisions and actions. Parents play an important role in establishing the inferiority complex in the female’s childhood exposure. Nora’s father is an essential initiator of Nora’s perception of the male superiority since her childhood, thus she grew up with the knowledge that the female has to be a male’s subject (Ibsen 104).  Ã‚  

Case study - Financial Report on Candescent Partners buying Physician

- Financial Report on Candescent Partners buying Physician practices - Case Study Example Moreover, this investment approach is beneficial for the company to focus more on business expansion and thereby get closer to its long term business goals. Introduction Candescent Partners, LLC is an investment/management company specialized in business expansion activities like buyouts, growth capital, middle market, mezzanine debt investments, and recapitalization. The company was founded by Steve Jenks and Sandy McGrath in 2009 at Boston in the United States. The company is very interested to invest in healthcare, IT sector, software services, consumer products, and business and technology enabled services. Candescent Partners is exploring investment opportunities in US and Canada based companies. As per its investment policy, the company’s investments may range from $2 million to $30 million and the firm tends to invest in companies having a revenue range of $10 million -$50 million, EBITDA between $2million and $10 million, and an enterprise value between $10 million and $75 million (â€Å"Company Overview of Candescent Partners†). Candescent Partners maintains a board seat in companies where it has investments. It is interesting to note that the organization raises funds for investments through personal capital. This paper will focus on the healthcare sector investments of the Candescent Partners by giving specific reference to the financial aspects of buying physician group practices such as Koch Eye in RI. Candescent Partners’ Investments in Healthcare Currently, the organization has managed an amount worth over $600 million of private equity investments in 44 private growing companies (Candescent Partners website). In this, a notable percent of private equity investments is accounted for healthcare technology and services. Some of the major physician practices bought by the firm are described below. 1. Koch Eye Associates Koch Eye Associates, one of the leading providers of comprehensive vision care services in Rhode Island, was a cquired by Candescent Partners in January 2012. Candescent Partners led this business acquisition in association with other co-investors including Pine Street Capital Partners, First New England Capital, Bush O’Donnell Capital Partners, and BCA Mezzanine Fund, LP. The Koch Eye Associates possesses its own surgery center and offers vision care services ranging from basic optometry to LASIK and cataract surgery. In addition, the organization

Withholding Information Essay Example for Free

Withholding Information Essay It is frequently advisable for those in positions of authority in government to withhold information from general public Some information the public has the right to know while other information is better to be withheld. There are plenty of reasons why it is better not revealing information. Withholding information that may put the population in danger would be better than revealing information which would bring chaos. On the flipside, transparency and honesty between the government and the public are very important. Transparency is essential in order for a democracy to function and honesty is important in cases when there is need for strong relations to be created. And although I support the idea of freedom of information, the general public will never be educated enough to maintain consensus and national problems. In these cases withholding information helps to ensure stability in terms of national security, preserving the peace and well-being. The main reason authority keeps information is the fear from criticism and disapproval. A rational society would never be satisfied with vague information and would want to take part in the decision-making process. Government has a lot of information which the people may have an interest in but still some of this information is dangerous. Things like war strategies and secret missions need to be withheld. Being kept in the dark on some things as long as its truly for the good of the nation and not for someone’s benefit is acceptable. For example if government find the cure for cancer but decide that it would ruin pharmaceutical companies and chose not to reveal this information it isn’t right. On the other hand, there is a lot of information which public doesn’t deserve access to. Like information that endangers the government. Another reason for withholding information could be to cover mistakes and not causing discontent. The government always makes big promises and eventually it will have to fulfill some of them. If government promise to reduce the crime rate in 30% within a year and don’t follow the plan it will lose it’s credibility. I think that the idea some information to be withheld is valid. However, information that would improve the living standard of people or information which is of great importance for the future of humanity, in my opinion shouldn’t be kept. There is no clear moral answer here. When there is too much that is at stake, we can’t say that information should always be free. Should it benefit the public to know how their government works, or it should benefit the government to have the full control over our lives. Im not so sure.

Telecommunication Trends - Fiber Optics :: essays research papers

FIBER OPTICS IN OUR SCHOOLS Fiber optic refers to the medium and the technology associated with the transmission of information as light impulses along a glass or plastic wire or fiber, about the thickness of a human hair. Fiber optic wire carries much more information than conventional copper wire, and is far less subject to electromagnetic interference. A single glass fiber can carry the equivalent of 100 channels of television or 100,000 telephone calls, with even more capacity possible by encasing many fibers within one cable. Fiber optics was developed by Bell labs and Corning in the late 1960s. It does not experience signal degradation over distance as would coaxial cable. School districts are aware of the need for the upgrades to fiber optic cabling, but costs frequently preclude the upgrade. Federal Communication Commission (FCC) rulings in October of 2004, that relieved incumbent local exchange carriers from having to share fiber networks that reach within 500 feet of homes, have led to plans by BellSouth to boost fiber deployments. Concern among competitors is that their ability to compete for business voice service will be hurt. (Quesada, 2004). But while the unbundling protection for fiber-to-curb is a blow to competitive local exchange carriers, BellSouth plans to increase deployments of fiber-to-the-curb by 40 percent in 2005, a move that will help decrease the cost for local school districts. Since any installation of new wiring is labor-intensive and costly, it is little wonder that school districts lag behind the corporate world in obtaining this superior technology. School districts are rarely provided with enough state and local funds to expand current technologies to encompass such upgrades. There are numerous programs and opportunities in place, however, that will allow even less affluent school districts to keep pace. One such program is called the Universal Service Fund for Schools and Libraries, or more simply, â€Å"E-rate†. E-rate was created by the Telecommunications Act of 1996, and is the discounted rate that schools and libraries pay for access to affordable telecommunications services. It was passed with the help of such politicians as Bill Clinton, Al Gore, and Newt Gingrich. The Act gave the Federal Communications Commission the power to expand universal access so that schools and libraries would be Internet wired. (Carolan & Keating, 1999). Since then, the trend to sign up for E-rate has swept the country. In 1999, Merrick and North Babylon students in Long Island, New York, discovered internet access when their schools opened, and Massapequa voters approved a multimillion-dollar bond issue that included Internet connection costs. Telecommunication Trends - Fiber Optics :: essays research papers FIBER OPTICS IN OUR SCHOOLS Fiber optic refers to the medium and the technology associated with the transmission of information as light impulses along a glass or plastic wire or fiber, about the thickness of a human hair. Fiber optic wire carries much more information than conventional copper wire, and is far less subject to electromagnetic interference. A single glass fiber can carry the equivalent of 100 channels of television or 100,000 telephone calls, with even more capacity possible by encasing many fibers within one cable. Fiber optics was developed by Bell labs and Corning in the late 1960s. It does not experience signal degradation over distance as would coaxial cable. School districts are aware of the need for the upgrades to fiber optic cabling, but costs frequently preclude the upgrade. Federal Communication Commission (FCC) rulings in October of 2004, that relieved incumbent local exchange carriers from having to share fiber networks that reach within 500 feet of homes, have led to plans by BellSouth to boost fiber deployments. Concern among competitors is that their ability to compete for business voice service will be hurt. (Quesada, 2004). But while the unbundling protection for fiber-to-curb is a blow to competitive local exchange carriers, BellSouth plans to increase deployments of fiber-to-the-curb by 40 percent in 2005, a move that will help decrease the cost for local school districts. Since any installation of new wiring is labor-intensive and costly, it is little wonder that school districts lag behind the corporate world in obtaining this superior technology. School districts are rarely provided with enough state and local funds to expand current technologies to encompass such upgrades. There are numerous programs and opportunities in place, however, that will allow even less affluent school districts to keep pace. One such program is called the Universal Service Fund for Schools and Libraries, or more simply, â€Å"E-rate†. E-rate was created by the Telecommunications Act of 1996, and is the discounted rate that schools and libraries pay for access to affordable telecommunications services. It was passed with the help of such politicians as Bill Clinton, Al Gore, and Newt Gingrich. The Act gave the Federal Communications Commission the power to expand universal access so that schools and libraries would be Internet wired. (Carolan & Keating, 1999). Since then, the trend to sign up for E-rate has swept the country. In 1999, Merrick and North Babylon students in Long Island, New York, discovered internet access when their schools opened, and Massapequa voters approved a multimillion-dollar bond issue that included Internet connection costs.

Professional Development Plan Essay

My professional plan is to grow as a nurse by gaining knowledge through school and my daily experiences, so in five years I can care for patients and families as a nurse practitioner. I will make short term and long term goals to help guide me down my path as I transition into the role of professional nurse; providing me with new opportunities for my future. Beliefs and values influences the decisions people make in their personal and professional life. My beliefs and values were incorporated to make my philosophy of nursing, that is to be an advocate for my patients’ and their family during their most vulnerable time, continuing my education to provide the best quality care available and taking a holistic approach by looking at the person as a whole; caring for their body, mind, and spirit (Chitty, 2007 p. 312). I believe nursing is an evidence based practice, the care provided to patients and family have been proven through research to be the safest most effective way to implement care. My values which mold my beliefs have been acquired throughout my life from the people around me and my experiences. My values and beliefs will continue to change as I get older, have more experiences in my profession and further my education in nursing. One of my short term goals is to finish my baccalaureate degree in nursing. In terms of advancing professionalism, research was recently published, (Morris & Faulk, 2007), in which nurses were found to have increased professionalism and growth in roles and values after attending an ADN to BSN program. That is what I am striving to achieve while going through the BSN program. I plan on taking each class one at a time, trying to do my best, and gain the knowledge needed to grow in my field. By August 2009, I should have my BSN which will provide me with new opportunities such as management and educator positions; this degree will also provide me the opportunity to further my education to a master’s level. My second short term goal will begin this summer, that is to initiate the evidence based practice, EBP, project on my progressive care unit, PCU, at Banner Good Samaritan Medical Center, BGSMC. Several of my coworkers and I are currently gathering information on research that suggests that providing patients and their families with written and oral information on the side effects of their medications will improve patient satisfactory scores in a acute care setting. We meet together regularly, learning as a team how to initiate EBP into the workplace. Then possibly in the future we will publish our findings. By fall of 2009 I plan on starting the Masters of Science in Nursing/Family Nurse Practitioner program at the University of Phoenix so I will be able to sit for the national boards certifying exam by the end of 2012. Once I complete the requirements I must submit the documentation to the Arizona State Board of Nursing. I then plan on working for an interventional cardiologist, which will give me the opportunity to work in both the hospital and office setting. Income is not the sole reason for my desire to advance in my profession but it is important to note that the U.S. Census Bureau 2000 data show that income levels rise as educational levels rise (Katz, Carter, Bishop and Kravitz, 2004). Income will enable me to continue my education and to obtain other goals in my future such as going to law school and traveling around the world. When discussing a professional development plan people have to keep in mind a persons support system, resources available to them, their work ethics and what is needed to succeed. While one may be able to fulfill his or her goals without one or more of these things, adversities can deter many from following plans they set forth. Support and understanding from my family and friends would be a significant tool in helping me achieve my goals. It may also be useful to have mentors help to guide me along my chosen path to success. Financial assistance will also be needed to continue on with my learning process. Self-discipline, further education and experience are also required to be successful in my future role as an FNP. Self-discipline and time management skills are crucial to have in our fast pace speed of lives’ today. It becomes increasingly important in the students’ life to use time wisely. Students may have to overcome several hurdles and barriers along the way. It is important to be able to identify these issues; it is the first step to overcoming them. Barriers may be financial, time restraints or lack of support from family and friends; having the knowledge how to overcome these barriers and having backup plans is what makes the difference between meeting your goal versus abandoning the goal. If faced with any of these barriers I will try to have a positive outlook and attitude, I will try to look on the bright side. Michaud (2002) observed that one’s ability to bounce back from disaster and loss depends on the person’s ability to use resiliency skills to conjure up positive emotions when he or she is challenged severely. I will monitor my stress level and use relaxation techniques specific to me and my needs if necessary in order to avoid abandoning the goals that I have set. In conclusion, putting together a professional plan is an important step to obtaining desired outcomes. According to Alexander Graham Bell (2001) â€Å"before anything else preparation is the key to success†. Values and beliefs will change over time, which will influence your original plan to formulate new goals, when this occurs a person must revaluate the resources available and challenge themselves to meet new goals. I will evaluate my plan to ensure I am staying on task meeting my goals that I have set forth so I will be successful in my future. Reference Bell, A. G. (2001). _Quoteland._ Retrieved March 15, 2008, from http://www.quoteland.com/topic.asp?CATEGORY_ID=447 Chitty, K. K. (2007). _Professional Nursing Concepts & Challenges (5th ed.)._ St. Louis: Elsevier Inc. Retrieved March 15, 2008, from University of Phoenix Library. Katz, J., Carter, C., Bishop, J. & Kravitz, S. (2004). _Keys to Nursing Success (2nd ed.)._ Upper Saddle River, NJ: Pearson Prentice Hall. Michaud E. (2000). There’s a secret to recovering from great adversity. The scientists who study it, and the people who’ve done it, share it with you. _Prevention Magazine_ (June). Retrieved March 15, 2008, from Proquest database. Morris, A., & Faulk, D. (2007). Perspective transformation: Enhancing the development of professionalism in RN-to-BSN students. _Journal of Nursing Education,_ 46(10), 445-51. Retrieved March 15, 2008, from Ebscohost database.

America Essay

It has been taught in American schools that the war fought in order to stop slavery and that the American South did the right thing by seceding from the Union because of its support in slavery. Subsequently, the Civil war was a result of the Founding Fathers’ failure to address the institution of slavery in a republic that proclaimed in its Declaration of Independence that all men are created equal. Nonetheless, the necessity of the war has been questioned because the four-war to abolish slavery ravaged the country and destroyed nearly half of the American people. Among the countries that practiced slavery, only America and Haiti freed their slaves by war. The rest were freed peacefully. Hence, it can be concluded that the South could have gained its independence without the war. The rising alone of keeping and posting guards on the constantly escaping slaves would have resulted in the gradual decline for the demand of slaves. The absence of a fugitive slave laws in other states that would force the return of the slaves to their owners has added to the decreasing value of the slaves. As such the institution would have been unsustainable regardless of the moral and humanitarian perspectives. On the other hand, the American North would still have achieved its goals without resorting to war. Apparently, the North slaughtered a large number of American young men in the battles against the South. Nonetheless, it is evident that the South posed no military threat to the North. Its aim of preserving the Union was considered as not a compelling reason to continue the war. The North wanted to keep the Union intact solely on the basis of economy and fiscal matter. Since the principal source of tax revenue for the federal government before the Civil war was a tariff on imports, they needed to keep these tariffs in which the federal government accounted its federal budget. The North did not need to take the bait by Lincoln who dispatched southerners to forestall the supplies for Fort Sumter. Hence, its attack on the South could have been prevented had not depended on the Union being intact and the economic advantages it provided them.

Best Part Time MBA Programs Essays

Best Part Time MBA Programs Essays Best Part Time MBA Programs Paper Best Part Time MBA Programs Paper Best Part Time MBA Programs  as well as accelerated masters programs can be completed in anywhere from four years all the way down to one year working full time. The part-time programs take a bit longer allow the flexibility of the student to maintain a full time job and/or care for his or her family while pursuing an advanced education. The first university on our list of the best part time MBA programs isNorthwestern  University. The 5  Best Part Time MBA Programs Simply click any of these schools corresponding banners below to be taken directly to the associated University program. 1)  Northwestern University  Part Time  MBA Program Overview   Business school at Northwestern University can be completed as quickly as one year. However there are other programs that offer flexibility depending on these students situation or educational requirements. Northwestern also has a two-year program that can be worked part time on Saturdays and in the evenings with a flexible class schedule. Students can also pursue joint degrees like an accelerated MBA/JD in as little as three years or up to four years with a PhD. in sociology as well as organizations and management at WeinbergCollege. NorthwesternUniversityis definitely one of the  best part time MBA programs  available they are a class leader in advanced education with world-class faculty and facilities to foster a healthy learning environment with great results that will translate into a better future for its alumni. Kellogg school of management additionally offers a two-year program to earn a joint degree of a Master of engineering management and an MBA degree. These courses focus on exceptional learning as well as teamwork and include a course in global studies that is also mandatory. The acceptance rate atNorthwesternUniversityis just under 50%. 2)  University of Chicago Part Time MBA Program  Overview The Booth business school at the University in Hyde Park Illinois is literally a hub of culture and arts that is right next to downtownChicagoandLake Michiganwith beautiful beaches. An MBA can be earned part-time or through the traditional two-year program via evening and weekend classes. The University of Chicago in conjunction with the booth school of business also offer joint degrees in various capacities as well as an executive MBA program which can be completed in just under two years. Our MBA program students use their instincts and competitive nature outside the classroom in various campus activities including volleyball games, three-legged races and tug-of-war challenges. theUniversityofChicagosees just over 1500 part-time enrollments of their MBA program per year. 3)  University of California Berkeley  Part Time  MBA Program  Overview Students at the   University of California Berkeley will enjoy the schools very unique location right by San Franciscowhich is an international hub for large businesses like Visa and Google. This area offers the convenience and opportunity of jobs and internships to assist with your advanced education. our students are assigned projects to both global and local companies and in about 21 months can earn their MBA degree via the traditional program. If a student would like to go through the part-time program using Saturdays and evenings for class time the masters degree can be had in about three years. More than half of the NBA is made up from elective this students can choose various courses at our facility and or both on campus or other divisions of UC Berkeley. For those ambitious students they have MBA programs on the east and west coasts. At UC Berkeley international students make up to a third of our MBA enrollments so many of our business classes are of global focus. 4)  New York University Part Time MBA Program Overview Business school students at New York University are grouped in classes of about 60 using these groups will shape their two years of graduate school education while fostering friendships and classmates as well as eating lunches together, taking courses together and competing in annual events. Students can choose from a broad range of electives across New York Universitys highly-ranked school system. An MBA in general management is a stern degree and students can choose to specialize in several areas such as luxury marketing, data as well as models and decisions. They also offer dual degrees and accelerated programs with the JD/MBA that takes about four years at theNYUUniversity. While an biology/MBA otherwise known as an MS will take about three years at the arts and sciences graduate school. An MS/MBA in finance and mathematics will take about three years at the Institute of mathematical sciences. These are just a few of their dual degrees that come with a part-time component. 5)  University of Michigan Part Time MBA Program Overview The University of Michigan business school program is an action-based program designed specifically to encourage its students to ask relevant yet important questions, identify specific problems and groom its students to become effective leaders. The university offers several MBA or executive MBA programs with a part-time MBA, full-time MBA and executive MBA degrees while working on weekends and evenings with the EMBA courses meeting just monthly. They also have degree programs for master of supply chain management as well as master of accounting and a PhD program. University of Michigan students can get involved in various institutes and centers at school including the global sustainable enterprise Institute with more than 70 clubs and organizations as well as the womens initiative that offers programming and counseling to women at the University. Stay tuned for our next article on the Economist MBA rankings and for now we hope you enjoyed our top five  best part time MBA programs  and remember if you have anything you would like to add or simply want to chime in maybe offer another school in the lineup we would be happy to hear from you as well as make any recommended additions to our best part-time MBA programs lineup.

Examples of Plural Tantum in English

Examples of Plural Tantum in English Plurale tantum is a  noun that appears only in the plural and doesnt ordinarily have a singular form (for example, jeans, pajamas, tweezers, shears, and scissors). Also known as a lexical plural. Plural:  pluralia tantum. Jeans, scissors, trousers, and glasses are great examples of plural tantum  nouns in the English language. Singular Tantum A noun that appears only in the singular formsuch as dirtis known as singulare tantum. Etymology of Plural Tantum Latin  for plural only Examples and Observations Richard Lederer [in Crazy English, 1990] asks, Doesnt it seem just a little loopy that we can make amends but never just one amend; that no matter how carefully we comb through the annals of history, we can never discover just one annal; that we can never pull a shenanigan, be in a doldrum, or get a jitter, a willy, a delerium tremen, a jimjam, or a heebie-jeebie? Lederer is alluding to pluralia tantum: Nouns that are always plural. Because they are not the result of pluralizing a singular, the complete plural form, -s and all, has to be stored in memory. Pluralia tantum in a sense are irregular regulars, and indeed they are happy to appear inside compounds: almsgiver (not almgiver), arms race (not arm race), blues rocker (not blue rocker), clothesbrush, Humanities department, jeans maker, newsmaker, oddsmaker, painstaking.(Steven Pinker, Words and Rules. Basic Books, 1999) Items of Clothing Lets take a look at other pluralia tantum in the pants/trousers family:(Mark Liberman, Language Log, Feb. 15, 2007) Outergarments: pants (orig. pantaloons), trousers, slacks, breeches/britches, bloomers, jeans, dungarees, bell bottoms, chinos, tights, shorts, trunks, Bermudas (extended to brand names: Levis, 501s, Wranglers, Calvins)Undergarments: underpants, long johns, skivvies, drawers, panties, knickers, boxers, briefs, undies, tighty-whities (extended to brand names: BVDs, Fruit of the Looms, Jockeys) How to Turn Lexical Plurals Into Count Nouns Nouns for articles of dress consisting of two parts are also treated as plural: [A] Where ​are my trousers?[B] They are in the bedroom where you put them. But such plural nouns can be turned into ordinary count nouns by means of a pair of or pairs of: I need to buy a new pair of trousers.How many pairs of blue jeans do you have? (Geoffrey Leech and Jan Svartvik, A Communicative Grammar of English, 3rd ed. Routledge, 2013) Lexical Concepts, Not Linguistic Classes The definitional property of having no singular turns out to be shallow and sometimes accidental, often (as in English) practically impossible to define and circumscribe. The state of affairs resembles the status of the mass-count distinction. . . . While they remain necessary as descriptive concepts, mass and count cannot be defined as grammatical properties of lexical items outside of a context, as Borer (2005) cogently shows. In the same way, I think, pluralia and singularia tantum are indispensable descriptive concepts, but they are not genuine linguistic classes. Therefore, we cannot build a notion of lexical plurals around that of pluralia tantum.(Paolo Acquaviva, Lexical Plurals: A Morphosemantic Approach. Oxford University Press, 2008)

Analyzing Market Segment Opportunities Essay Example | Topics and Well Written Essays - 750 words

Analyzing Market Segment Opportunities - Essay Example Recreational Tennis Players play tennis for fun with their families and friends, say at clubhouses, at homes, at schools, and other private tennis instillations. Therefore, these players do not have the ability to go for the most professional racquets for playing the game. Most of them merely settle for any racquet that can enable them play and enjoy the game. However, other recreational players are staunch lovers of the game. They do not play tennis professionally because they have other priorities in their lives, e.g. as professionals in other fields such as medicine, business and finance (Lipsey, 2006). Nevertheless, these players put all their efforts during free times to play tennis both professionally, i.e. in small local tennis leagues or with friends, and for fun. Consequently, they are more particular about the racquets they while playing tennis. In this case, they are always willing and able to part with a significant amount of their income to purchase a good tennis racquet . Prince Incorporation can reach out to these recreational players through social media sites such as Facebook and Twitter. Social media sites are the most common place where these players converge to read news about their favorite tennis players and their performance in various league games. They prefer emulating their ‘tennis idols’ by using similar racquets as those used by professional players (Babin & Zikmund, 2012). (b) Junior players Junior tennis players are those players who are still in colleges and high schools. These youngsters have a passion of playing tennis in future whether professionally or otherwise. They have tennis idols who are professional tennis players whom they look up to as their role models and hope to be like them when they grow up. This is the stage where most youngsters develop their talents in playing tennis. However, the only hindrance for these players in exploiting their passion for tennis is the fact that they cannot afford to purchase the tennis racquets they fancy or wish they could have. This is because most of them cannot accumulate enough savings from their pocket money to purchase these racquets. In addition, their parents or guardians might not be willing to part with significant amount of money to buy them a professional racquet, especially if they are not that talented (Stevens, 2012). As such, professional racquet companies such as Prince Incorporation can reach out to this segment of tennis players either through their parents or through their schools and colleges. They can collaborate with schools as well as other learning institutions to sponsor inter-school tennis competitions, or set up promotions for young tennis lovers within learning institutions. This will enable them reach out to a large number of students, i.e. the junior players, who will get to know of the existing racquets on offer by the company and how much they cost. In fact, they may even get to test some of these racquets during these promotional drives. Apart from schools and learning institutions, these companies can also market their products through established companies where parents and guardians of these children work, or spent their social time. In addition, broadcast media also provides a lucrative platform for these companies to reach out to their clients (Lipsey, 2006). What might Prince do to help it gain distribution and sales in (a) Mass merchandisers like Target and Wal-Mart Mass merchandisers of tennis racquets such as Wal-Mart and Target already have a brand name in which they trade in. This brand name enables these stores to market their pro

Audit of School Community Resources and Supports Assignment

Audit of School Community Resources and Supports - Assignment Example This makes the school administration to document the resources that it has and identify the gaps and weaknesses. Using this information, it can devise viable ways through which it can source for important resources. It is against this background that this paper provides an audit of the school in light of the community services and supports. To ensure a coherent consideration, it analyses the relative strengths and gaps in a bid to underscore the extent of these inconsistencies to the performance of the school and improvement of student behavior. Activities Conducted During the Audit The researcher spoke to about 50 individuals to determine the type of resources and support systems that were in place as well as their importance to the school. This also sought to determine the resources that were missing and what would be done to ensure that they are sourced. Further, the researcher interviewed the students to determine the manner in which they benefit from the available resources. The interviews were aimed at establishing whether the students are comfortable with the available resources and what they think should be done to improve performance. The researcher then spoke with the key stakeholders in the industry and specifically, those that were directly involved in the support system. These ranged from the school administrators such as the superintendants, teachers, chief academic officers, parents and a host of other education officials. Further, the researcher conducted a survey of the fifth grade students to determine whether they feel both physically and emotionally supported, safe, challenged or connected. Underlying efforts in this regard were also geared towards underscoring the nature of the learning environment and to what extent it encourages, inhibits or and supports social responsibility. Finally, the researcher reviewed the reports that had been previously made regarding the current support systems, their impacts on the behavioral wellbeing of the s tudents as well as their contribution to the academic performance of the students. The preceding activities had insightful views about the state of the support system and its contribution and influence on the overall performance of the students. They identified the support systems and services currently available and those that are missing. Most importantly, the findings underscored the strengths and weaknesses of each. At this point, it is worth noting that the preceding information is vitally important. Essentially, Scott and Hunter (2001) assert that it would act as a basement upon which critical decisions would be made. In particular, it would be important to positively emphasize the strength and eliminate or discourage the weaknesses. This way, the support systems and services would yield desirable outcomes. Critique of Strengths and Gaps One of the strengths that the institution has pertains to the active parent involvement in the education of the students. In this regard, the parents are well informed about their roles in the learning process. They attend all the meetings that are geared towards addressing the challenges that the students face. In addition, there is a cordial relationship between the teachers and the parents. In this respect, Lawson and Sailor (2000) assert that active involvement of the parent in the life of the student enables the former to understand the challenges being faced by the

Outsourcing and Offshoring Essay Example | Topics and Well Written Essays - 2000 words

Outsourcing and Offshoring - Essay Example The changes at WW Company are in a bid to achieve 5% economic growth and cut down costs by 5%. The company believes that an incorporation of information technologies will enable it perform competitively in the transportation and distribution industry. In the restructuring process, WW has identified an area in service delivery and customer relationship that would highly benefit the company in achieving its goals. Firstly, the company has identified its existing operational systems as slow and duly lacking in optimal service delivery. Employees in various departments complain of too much work load in accurately feeding the systems with data. They say that the system is error prone due to the fact that most of the input work is done by the employees who are likely to make inaccurate entries. Additionally, employees complain that the existent systems are too slow in execution of tasks, thereby causing a hold up in work management and service delivery. The new changes in IT have seen a range of projects span up at WW. Among the projects includes the development of a Customer Relationship Management Software. Right before the introduction of the changes at WW, the management had a meeting where they decided to change the company’s strategic plan. The changes in strategic planning were essential in that they would suit the new direction that the company was taking, which involved incorporation of information technology. With the introduction of IT at WW, all systems would go upgrades and re-specification so as to ensure that they perform as expected. Additionally, the newly hired Chief Information Officer of WW was expected to introduce technological systems in areas where they had not yet been explored in the company. This includes the newly proposed Customer Relationship Management Software. The proposed project involves

The Integrated Curriculum Model Education Essay

The Integrated Curriculum Model Education Essay William and Mary Center for Gifted Education Research Model, the Parallel Curriculum Model, and the Integrated Curriculum Model are advanced, progressive curriculum models, which contribute to a consistent improvement of the learning process, engaging students in learning and encouraging them to learn more and more successfully. In this regard, principles and components of these models are very important for the overall success of the learning process. At this point, it is important to place emphasis on the fact that William and Mary Center for Gifted Education Research Model attempts to apply a combination of the Parallel Curriculum Model and the Integrated Curriculum Model. On analyzing, the three models, it is possible to distinguish some common principles and components. First of all, the three curriculum models focus on the self-development of students, which is extremely important today, when students are overwhelmed with a huge amount of information and they need to process information to obtain knowledge and generate new ones. What is meant here is the fact that modern students cannot rely on their educators solely. Instead, they should focus on self-development and this is exactly what the three curriculum models are concerned with. Students obtain a lot of information from the outside world and they have to be able to process the information to accumulate knowledge and to generate new knowledge on the ground of acquired one and their own experience. As a result, students, prove to come prepared to the life in the modern society, where information rules. Furthermore, all three models focus on the development of engaging curriculum. According to the three models, the main purpose of the curriculum is not only to help students to acquire new knowledge, develop new skills and abilities, but also to be engaged in the learning process. The latter means that students should be interested in the learning process and the three models focus on the engagement of students because a profound, sincere interest of students to the learning process increases the effectiveness of learning consistently. In this regard, the high motivation of students is another fundamental principle the three models follow strictly. The three models attempt to reach the high level of motivation through non-material encouragement of students. They focus on the revelation of good perspectives of personal and professional development for students. In addition, these models provide students with a positive feedback from their educators for any successes students reach in the course of their learning. At the same time, William and Mary Center for Gifted Education Research Model and the Integrated Curriculum Model focus on the use of the multidisciplinary approach, whereas the Parallel Curriculum Model tends to the intradisciplinary approach. The multidisciplinary approach leads to the close interaction between different disciplines. In practice, this means that students have to use their knowledge, skills, abilities and experience from different disciplines in the course of learning. For instance, while performing a task on history, they may need to use their knowledge of geography, language, mathematics, and other disciplines. The Parallel Curriculum Model tends to use the intradiscipline approach, which focuses on specific disciplines and helps students to expand their knowledge to focus on specific field, which are particularly interesting to them maintaining the correlation to other disciplines. Furthermore, William and Mary Center for Gifted Education Research Model and the Integrative Curriculum Model, in a way, focus on the use of art in the learning process. In this regard, William and Mary Center for Gifted Education Research Model is particularly concerned with the use of art. The use of art is closely intertwined with the aesthetic development of students, which is another concern of William and Mary Center for Gifted Education Research Model and Integrated Curriculum Model. In this respect, it is worth mentioning the fact that art and aesthetic development of students are extremely important in the contemporary society because students live in the highly materialistic world. The emerging consumerism leaves little room for the aesthetic education and art in the life of students. In such a situation, education should offer students an opportunity to learn and to understand art, to develop the non-material, aesthetic side of their personality. The use of art is particul arly useful for potentially gifted students. Therefore, William and Mary Center for Gifted Education Research Model uses art to reveal gifts of students and to help them to realize their full potential. Finally, all the three models use humanistic principles, which are also very important in the modern, consumerist society. In such a way, the three models attempt to return students to fundamental, humanistic principles, which lay the foundation to the modern western civilization. These humanistic principles are closely intertwined with democratic norms and principles, which are fundamental for the modern society. NEEDS ADDRESSED The three models pay a lot of attention to the development of critical and creative thinking. As the matter of fact, critical and creative thinking is essential for the extensive students personal development. Students receive a lot of information from the surrounding world but they have to evaluate the information critically to accumulate knowledge and to avoid fallacies, which they can fall in without critical thinking. At the same time, creative thinking, which is particularly important for William and Mary Center for Gifted Education Research Model contributes to the broad development of students values, beliefs, norms and standards. In addition, creative thinking helps students to develop skills of effective problem solving. In fact, William and Mary Center for Gifted Education Research Model encourages creative students to realize their full potential because it is through students creativity they can realize their individuality. At the same time, the three models maintain the differentiation of students. What is meant here is the fact that all students have a different level of academic development, different psychological traits and characteristics, different gifts and inclinations. Therefore, the three models tend to develop differentiated curriculum to meet needs and wants of each students. In this regard, the individual approach to students is particularly effective and the three models attempt to find the individual approach to each student. Furthermore, the enrichment of students is another concern of the three models, although the Integrative Curriculum Model and the Parallel Curriculum Model focus on the academic development of students and stress the importance of the practical application of knowledge and skills acquired by students in the course of learning, they still focus on the enrichment and broad development of students. Unlike the Integrative Curriculum Model and the Parallel Curriculum Model, William and Mary Center for Gifted Education Research Model focuses not only on enrichment of practical knowledge, skills and abilities of students, but also on the development of aesthetic values and creativity of students. In addition, William and Mary Center for Gifted Education Research Model focuses on the acceleration of students development to help them to realize their talents. In this regard, the Integrative Curriculum Model and the Parallel Curriculum Model avoid excessive acceleration of students development because such acceleration affects consistently the personal development of students. Often the accelerated development can become an unbearable burden for students, they become too concerned with their learning and they face problems with their socialization because of the lack of free time. In addition, the three models focus on social and emotional needs of gifted and talented learners. The three models view social and emotional development as an essential element of education. Students need to undergo the process of socialization and develop the balanced, harmonious personality. The three models focus on motivation of students. They encourage students to learning and increase their interest to learning. In this regard, questioning is an important part of educational process because the three models stand on the ground that students should always question (VanTassel-Baska Brown, 2000). Through questioning, they learn how to find and obtain knew knowledge independently of educators. Finally, the three models focus on the use of technology in the process of education. Today, technology affects all spheres of human life. Therefore, students should come prepared to learn using new technologies and to develop new skills and abilities. In this regard, education helps students to use technology in their everyday life. ADVATNAGES AND DISADVANTAGES Obviously, each of the three models has its own advantages and disadvantages. On analyzing advantages of each model, it is worth mentioning the fact that William and Mary Center for Gifted Education Research Model focuses on the development of talents of students. This model pays a lot of attention to the development of creativity of students. In addition, the model contributes to the creative development of students and to the formation of their aesthetic tastes, values, norms and standards. The Integrative Curriculum Model focuses on the use of multidisciplinary approach and contributes to the close integration of all disciplines (Avery VanTassel-Baska, 2001). As for the Parallel Curriculum Model, this model allows students to focus on the specific field they are interested in. On the other hand, each model has its own disadvantages. William and Mary Center for Gifted Education Research Model tends to overestimate the aesthetic development of students and individual approach to each student, whereas the Parallel Curriculum Model focuses on the practical aspects of the development, underestimating the significance of creative and aesthetic development of students. The Integrative Curriculum Model does not always allow students to focus on the narrow, specific field, which may be helpful for their future professional development. BEST MODEL On comparing the three models, it is possible to distinguish William and Mary Center for Gifted Education Research Model as the best model. Obviously, William and Mary Center for Gifted Education Research Model provides students with ample opportunities to realize their full potential. At the same time, educators can develop an individual approach to each student and develop their creativity. In such a way, educators can identify gifted students and develop their talents. Today, the creative development of students is essential because the highly technological society and information age raise the demand on creative professionals. In addition, William and Mary Center for Gifted Education Research Model helps to the personal development of students. As a result, this model contributes to the broad development of students, shapes their values, views, norms and standards. In fact, this model prepares students to the adult life and allows them to find their own way in the professional de velopment and in their personal life. Each student should be capable to realize his or her unique inclinations and capabilities.

Bilingual Education Essay -- essays research papers

Bilingual education was first initiated in 1968. It was a new means to educate the children who spoke a minority language. thirty-one years later the same problems exist for those children who speak a language other then English. The experiment of Bilingual education has been a failure and now it’s time to move on. The first English only initiatives were brought forth in 1981 by newly elected president Reagan. Since then the conflict over Bilingual education has drove on. Currently twenty-three states have â€Å"English Only† laws with 4 states having laws that are pending. The issue of bilingual education and the conflict that surrounds it is primarily focused between whites and Hispanics, although since the mid 70’s it’s also been involved with the Chinese and Mung cultures. Bilingual education has failed however, currently the movement is towards and English only educational system. The simple fact of the matter is that people who speak a language in th is country will never get ahead. We’ve tested the ignorant notion that you could get by without speaking English; or speaking English very minimally, but that’s promoting and even more ignorant idea which is that you don’t need speak English fluently to succeed in America. In 1968 we didn’t know that Bilingual Education wouldn’t work, however, it’s time to move on and do the right thing and switch to English Only.   Ã‚  Ã‚  Ã‚  Ã‚  Lyndon Johnson was president, we had the futile dream of world peace, at the same time we were â€Å"Bombing the North Vietnamese into the stone-age†. In the final year of LBJ’s presidency sever new education initives were taken as part of the last step in his â€Å"Great Society† programs. One of these new initives was Bilingual education. At the time it was supported by the Liberal whites in this country, for the most part the conservative Hispanic sect was opposed to such measures. The idea at the time was, kids would learn in their native language and simply pick up English gradually. Those ideas were radicalized in the 1970’s however, the premise moved away from gradual learning of English; to English wasn’t really necessary. In 1981 Bilingual education came under assault from newly elected president Reagan proposed moving to the English Only system. The idea wasn’t viewed as Anti-Hispanic at the time, or as some kind of racist proposal. Reagan was adored by a majority of th... ...and since 1908 printed exclusively in English by the United States Government. Teddy Roosevelt said in 1908 that â€Å"To print the American Constitution in any other language but English, would be like spitting in the face of our forefathers†. The Untied States Supreme Court has also ruled numerous times that English only laws do not violate the 1st amendment of our Constitution.   Ã‚  Ã‚  Ã‚  Ã‚  I personally believe that in our nation there needs to be a main language, not 3 or 4 or 1200. George Washington wasn’t giving orders at Valley Forge in Russian he was speaking English. Abe Lincoln didn’t give the Gettysburgh Address in Portuguese. It’s not to much to ask for every American citizen to be able to speak English fluently. No one is forced to live here, and if you refuse to adapt you probably shouldn’t be living here. If you want to speak German by all means go ahead and do so, but do it back in Germany. While you’re in America however, we speak English, and if you don’t know it you’re in trouble. The conflict is over rated. It’s not a matter of racism or of any other kind of hatred. It’s a simple matter of principle. American’s speak English it’s not to much to ask.

Favorite Family Get-Together

Getting together with family is something that most of us look forward to. Throughout the years our family has grown with all sorts of personalities, Making every get-together fun, interesting, and memorable. Even though I see my family quite often, our get-togethers are always quite entertaining. While we celebrate birthdays, holidays, special occasion, and new or old traditions, By far my three favorite are my mom's birthday, pumpkin carving contest, and New Year's.My first favorite family get together is my mom's birthday. No matter what day the week her birthday is my siblings and I always managed to gather our families enjoying my mom for dinner. It's indescribable how happy that makes her. We bring her favorite dish and pastry. At the dinner table we take turns to express our gratitude and I love for my mom. Throughout the night we gathered up and see her open the gift and read the card her grandkids made for her. Every year is a blessing to be able to enjoy that moment.My seco nd favorite family get together is our pumpkin carving contest. We came up with this tradition couple of years ago. Teams are formed by family and their kids, we help them carve their pumpkin into any character or picture of their choice. To make it fun we give out prices for the winning pumpkin. The children get so involved and become competitive that the competition gets quite interesting. The losers end up cleaning the mess left over from the carving process it became more popular with the kids as they grow older.The competition became more serious. But at the end of the contest everyone goes home with a great carved pumpkins. My third favorite family get together with the New Year's day. Our family celebrates not only for the upcoming new year But also for the year that ends. The children play throughout the night and the adults just relax, Conversation; Making it seem like we rarely see each other throughout the year. My family only consists of my immediate family.Making it mor e meaningful, private, and warming. The countdown to the new year is by far the best. We get in spirit, putting on hats, glasses, beads of the upcoming new year numbers. As a family we end the year and we welcome the new year with popping streamers, and loud horns, The most importantly as a family. Throughout the years our family get-togethers have become memorable. Celebrating birthdays traditional events and holidays makes our little families become one.

Dna Analysis Practical Write-Up

Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E. coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E. coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markersAbstract This work describes a lysis method for the isolation and purification of bacterial genomic DNA and visualization of the restriction fragments by agarose gel electrophoresis. It was noted that for one to isolate and purify bacterial chromosomal DNA several steps are taken into consideration. DNA was found to absorb at 260nm wavelength in a UV spectrophotometer. Restriction enzymes were added to cleave DNA which would produce various DNA fragments. DNA can be separate d into different sized fragments by gel electrophoresis.The bacterial DNA was successfully isolated and purified however it could not be observed after running the gel. DNA analysis is a standard practice for defining paternity or maternity, predisposition to disease, embryonic health and criminal guilty. But in our context, DNA analysis is mainly used for predisposition of diseases in bacteria. Bacteria are pathogenic microorganisms that cause infectious diseases including cholera, syphilis, anthrax and leprosy. The most common fatal bacterial diseases are respiratory infections such as tuberculosis (Barnum S.R; 1998). Nucleic acids encode information relating to cell structure and function. Cells have the ability to make copies of their DNA and pass this information to daughter cells. Nucleic acids are polymers of nucleotides. Nucleotides are composed of ribose (a 5` carbon) sugar and either a purine and pyrimidine base at 1` position. The purine bases are adenine (A) and guanine (G) and the pyrimidine bases are cytosine (C), thymine (T) and Uracil (U). Uracil is only found in RNA and thymine is only found in DNA (Wiser M. F; 2002).Isolation of nucleic acid – three major types of techniques are employed in the isolation of nucleic acids differential solubility, absorption methods or density gradient centrifugation. The choice of method will depend on the type of DNA being isolated and the application. A major goal of nucleic acid isolation is the removal of proteins. The separation of nucleic acids from proteins is generally accomplished due to their different chemical properties. In particular, the highly charged phosphate backbone makes the nucleic acids rather hydrophilic as compared to proteins which are more hydrophobic (Allison L.A; 2012). Spectrophotometry is a versatile analytical tool. The underlying principle of spectrophotometry is to shine light on a sample and to analyze how the sample affects the light. DNA absorbs light at a wavelength of approximately 260nm (Stryer; 2006). Centrifugation is a process that involves the use of the centrifugal force for the separation of mixtures. Separation is based size, shape and density. It utilizes density difference between the particles/macromolecules and the medium in which these are dispersed (Gupta P. K; 2006).Dispersed systems are subjected to artificially induced gravitational fields. A buffer is an aqueous solution consisting of a mixture of weak acid and its conjugate base or weak base and its conjugate acid. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent any change in the pH of a solution (Cowan M. K; 2009). Electrophoresis is a diverse technique of separation used to separate and sometimes purify macromolecules especially proteins and nucleic acids that differ in size, charge or conformation by an electric current (Stryer L. 2006). Gel electrophoresis refers to using a gel as an ant convective mediu m and or sieving medium during electrophoresis. Gel electrophoresis is most commonly used for separation of biological macromolecules such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein; however, gel electrophoresis can be used for separation of nanoparticles. Materials Used * Luria Broth medium * SET Buffer * TEN Buffer * Choloroform/isoamyl alcohol. 24:1 mixture * Phenol/ chloroform 1:1 (Buffer saturated phenol) * Ethanol (95%) stored at -20? * Na Acetate * NaCl: 5M sterilized by autoclaving Sodium dodecylsulphate (SDS) : 26% (w/v) * Bacteria cells * Plastic test tubes * Glass rods * Wide bore pipette * Ice bath * Centrifuge * Ethidium bromide * Agarose * TBE buffer Methodology Each group carried out the following procedures: Used two 50ml sterile plastic tubes, harvested cells by centrifugation for 10 min 4’C. Combined pellets to give approximately 1g wet weight of cells. Washed the pellet, re-suspended it in 20ml Ten buffer by gentle vortexing. Harvest ed the cells again as described above. Re suspended the cells in 10ml of Set buffer and let them sit on ice for 5min.Added 1000Â µL of lysozyme and incubated at 37? for 30 min. Divided the cell suspension into two in separate sterile 50ml tubes. Added 5 ml Ten Buffer and 500Â µl of SDS. Gently mixed the tubes by inverting them until lysis occurred. To each tube added 1ml 5M NaCl and an equal volume of buffer saturated phenol. The tubes were inverted till the mixture was emulsified. Separated the phases by centrifugation for 10min at 40C. Recovered the upper aqueous phase using a wide bore pipette. When retaining the aqueous phase the pellicle at the interface was avoided. Repeated the extraction until the interface was clear.Added an equal volume of chloroform and extract residual protein as described above. Transferred the upper aqueous phases from both tubes to a 100ml beaker. Set them on ice and added 1/10th volume 3M Na acetate. Precipitate the DNA by addition of 2 volumes of ice cold 95% ethanol. Mixed thoroughly and allow it to stand for about 5min on ice for the DNA to precipitate. Spooled the DNA out of solution on a glass rod, dipped it into a tube of 95% ethanol and re-suspended in 10ml Ten Buffer. Left to dissolve overnight at 4’C B) Gel electrophoresis The gel was prepared by melting 1. 6g of agarose plus 200ml of 0. x TBE buffer. Swirled the mixture and allowed it to cool to 55?. Added 10? l ethiduim dye Loaded the gel in the following order; 1. Undigested pBSK 2. pBSK + digested with Eco R1 and Xba 1 3. Undigested DNA from a blue colony 4. DNA from a blue colony digested with Eco R1 and Xba 1 5. Undigested DNA from a white colony 6. DNA from a white colony digested with Eco R1 and Xba1 7. Lambda Hind III molecular weight markers After loading the gel it was run at 100 volts for 2 hours. Results We managed to precipitate DNA out of the Bacterial cells. DNA was seen a small white like fragments.However we could not spool the DNA out of sol ution using glass rods due to fact that DNA is a fragile compound hence when we twisted / spooled for DNA we destroyed the DNA strands cutting them into smaller fragments. The following day, analysis of the DNA sample in a spectrophotometer was carried out. It was found that DNA absorbed a specific wavelength of 260nm. This proved the presence of DNA in the sample. Our sample was digested by restriction enzymes and labeled the DNA fragments with an identification dye and ran them on the Gel electrophoresis together with molecular weight markers.After running the gel no observeable bands of different band fragments were observed. Only the molecular weight markers bands were observed. Discussion The TEN and SET buffer were used to lyse the cells. They are good buffering agent, which solubilizes the DNA, while protecting it from degradation. Eluting and storing the DNA in TBE Buffer is helpful if the EDTA does not affect the downstream applications. EDTA chelates or binds to Mg2+ ions present in purified DNA and can help inhibit possible contaminating nuclease activity (Cowan M. K; 2009).Balancing of test tubes before centrifugation in order for the centrifugation process to be effective to create centrifugal field that results in maximum separation of cell components. According to Wiser M. F 2002, DNA is very insoluble in ethanol and isopropanol, but both alcohols are very water soluble. Thus, it will dissolve in water to form a solution and cause the DNA in the solution to aggregate and precipitate out. Isopropanol is often better to use because it has greater potency in precipitating the DNA and thus lower concentration is required. This is advantageous because it will take less time for the isopropyl alcohol to evaporate.Salts such as sodium chloride and ammonium acetate remove histone and non-histone chromosomal proteins bound to the DNA. As soon as 95% ethanol was added after sodium acetate for DNA precipitation, the whole solution turned cloudy with a lot of white precipitate, precipitating down. According to Allison L. A, 2012; sodium acetate which is negatively charged and low pH was used which contributes to charging positively the DNA. A combination of this plus high salt molarity enhances formation of aggregates of DNA and facilitates the pelleting procedure. Chloroform isoamyl-alcohol is a type of detergent.It binds to protein and lipids of cell membrane and dissolves them. By this it disrupted the bonds that hold the cell membrane together and cause it to breakdown. It then forms complexes with these lipids and proteins, causing them to precipitate out of solution (Besty T and Keogh J; 2005). This reduced chance of contaminated DNA being obtained hence making it possible for us to be able to precipitate DNA only. Alcohol (95%ethanol) is used to precipitate DNA. SDS which stands for ‘sodium dodecyl sulfate' is a strong anionic detergent that can solubilize the proteins and lipids that form the membranes.This will helped t he cell membranes and nuclear envelopes to break down and expose the chromosomes that contain the DNA. In addition to removing the membrane barriers, SDS helped release the DNA from histones and other DNA binding proteins by denaturing them (Barnum S. R; 1998). Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA (Wiser M.F; 2012). Molecular weight size is a set of standards that are used to identify the approximate size of a molecule run on a gel. These markers were composed of nucleic acids of different sizes. A few reasons you may not see bands on the gel after electrophoresis: When preparing the gel for electrophoresis TBE buffer was used. This was done so that the temperature can be maintained and lubricate the electrolyte. L oading dye was added this helped weigh down the DNA so that it can sink into the bottom wells and not float in the buffer solution. According to Gupta P.K, 2006; loading dye moves quickly than the actual DNA parts so it is an indicator to when to turn off the power on the electrophoresis chamber. The dye also makes the DNA visible to the naked eye, giving it a purplish color and making it easier to work with. After Gel electrophoresis no bands of DNA were observed. This according Allison L. A (2012) might have been as a result of any of the following * DNA concentration might have been too low. * DNA sample is contaminated with RNA and Protein * DNA bands are too small and have run out of the gel The buffer system in which the gel is suspended is not doing its job correctly. The buffer might have to be made fresh. * The electrophoresis apparatus is not in the correct orientation (electrodes not connected to the right poles). The major drawback in the experiment was that our fellow c olleagues were not able to isolate and purify their DNA. Also when working with DNA temperature regulations were not sometimes adhered to, it was sometimes left on the surface tables for long periods esp. when the samples were being analyzed in the spectrophotometer.Recommendations With proper teamwork and co-ordination among my fellow classmates much larger quantities of DNA could have been isolated and purified. The DNA should not be kept at room conditions for a long time. Conclusion The experiment was partly a success managed to isolate and purify DNA, analyzed it using a spectrophotometer. However bands of DNA could not be visualized after running the gel. References 1. Allison L. A. (2012). Fundamental Molecular Biology, 2nd edition. Denvers. John Wiley and Sons Inc. 2. Barnum Susan.R, (1998), Biotechnology: An introduction, New Delhi, Vikas Publishing House. 3. Besty Tom and Keogh Jim, (2005), Microbiology demystified, New York; MacGraw-Hill. 4. Cowan Majorie Kelly, (2009), M icrobiology: A Systems Approach, 3rd edition; New York; MacGraw-Hill. 5. Gupta, P. K. (2006). Elements of Biotechnology, Meerut. Rastogi Publications. 6. Stryer L, Berg J. M and John Tymozcko. (2006). Biochemistry. 5th edition. California. W. H Freeman and Company. 7. Wiser, M. F. (2002). Methods in cell biology. Berlin. Springer Verlog CHINHOYI UNIVERSITY OF TECHNOLOGYName Tanyaradzwa R Ngara Reg Number C1110934J Course Recombinant DNA Technology Module Code CUBT 203 Program Biotechnology Level 2:1 Lecturer Dr Mlambo Practical Write-up DNA analysis